wi 38 va13 subline 2ra cells  (ATCC)

Journal: Stem cell research

Article Title: Telomere dynamics and hematopoietic differentiation of human DKC1 -mutant induced pluripotent stem cells

doi: 10.1016/j.scr.2019.101540

Figure Lengend Snippet: (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in VA13 cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.

Article Snippet: Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13, from ATCC) were used as the positive control.

Techniques: Immunofluorescence, Clone Assay, Staining, Labeling, Positive Control, Dot Blot, Control

Journal: Phytomedicine

Article Title: GMI, a protein from Ganoderma microsporum , induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus

doi: 10.1016/j.phymed.2022.154215

Figure Lengend Snippet: GMI downregulated expression of ACE2 in WI38–2RA cells in vitro and lung tissue of mice in vivo . (A) The viability of WI38–2RA cells treated with GMI was evaluated. Each GMI-treated group was normalized by the control group. (B) The ACE2 levels of WI38–2RA cells treated with GMI were evaluated by Western blot. (C-F) WI38–2RA cells were pretreated with DMSO/ddH 2 O (vehicle control) or MG132 (10 μM; C)/Bafilomycin A1 (BafA1; 20 nM; D)/dynasore (200 μM; E)/MβCD (20 mM; F) for 30 min, followed by incubation with GMI (0.6 μM) for 24 h and their ACE2 expression levels were evaluated. (G) Scheme for mouse receiving GMI via inhalation method. (H) The expressions of ACE2 protein in lung tissue were determined by Western blot. Tubulin was used as internal control. Each number (#1, #2, #3) represents the data collected from one single mouse. The data were representatives of more than three separated experiments and were presented as mean ± SDs; the error bars indicated SD. Significant differences were noted (*** p < 0.001).

Article Snippet: The normal human lung fibroblast WI-38 VA-13 subline 2RA (WI38–2RA) cells were purchased from the Bioresource Collection and Research Center of the Food Industry Research and Development Institute (BCRC, Hsinchu, Taiwan).

Techniques: Expressing, In Vitro, In Vivo, Control, Western Blot, Incubation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Additive Antiproliferative and Antiangiogenic Effects of Metformin and Pemetrexed in a Non-Small-Cell Lung Cancer Xenograft Model

doi: 10.3389/fcell.2021.688062

Figure Lengend Snippet: Additive cell cycle arrest and apoptosis effects of metformin and pemetrexed on the NSCLC and normal lung cell lines. Flow cytometric analysis and the cell cycle distribution of (A) A549, (B) H1975, (C) HCC827, and (D) WI38-2RA cells treated with metformin (0.2 mM) and/or pemetrexed (1 μM) for 48 h. ( A–D , right panel) Quantification of cell cycle distribution. (E) Cytotoxicity of A549, H1975, HCC827, and WI38-2RA treated with the indicated concentration of metformin and/or pemetrexed for 48 h as assessed by flow cytometric analysis with Annexin V/propidium iodide (PI) staining. Percentages of Annexin V-positive cells (apoptosis cells population) are shown. The data are presented as the mean ± SD of those from three independent experiments with three technical replicates in each. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the Ctrl group; # P < 0.05, ## P < 0.01, and ### P < 0.001 compared with the Met group; & P < 0.05, && P < 0.01, and &&& P < 0.001 compared with the Pem group.

Article Snippet: Normal human embryonic lung cell line WI-38 VA-13 subline 2RA (WI38-2RA; BCRC-60504) was purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan).

Techniques: Concentration Assay, Staining